Fig. 4

miR-663a targeted AP-1 component JunD in NSCLC cells. a In H460 cells transfected with miR-663a mimic, the AP-1 activity was lower than that in H460 cells transfected with control. The AP-1 activity in H1299 transfected with miR-663a inhibitor was higher than that transfected with control. b JunD protein and mRNA expression in H460 cells treated with miR-663a mimic was lower than that in H460 cells transfected with control. JunD protein and mRNA expression in H1299 treated with miR-663a inhibitor was higher than that in H1299 cells transfected with control. c Wild-type and mutant miR-663a binding sites in the 3′-UTR of JunD were assessed using fluorescent reporter assays. The fluorescence activity of miR-663a mimic/mimic control was calculated. In H460 cells transfected with reporter vector containing wild-type binding site, the luciferase activity in cells with miR-663a mimic was lower than that in control cells. d JunD siRNA treatment in H1299 cells downregulated protein and mRNA expression of cyclin D1, cyclin E and MMP9. In JunD depleted cells, difference of cyclin D1, cyclin E, MMP9 mRNA in cells treated with control and miR-663a inhibitor was not significant. Experiments were repeated in triplicate. *p < 0.05, Error bars indicate standard deviation