Fig. 2

Effects of miR-663a on proliferation and invasion in NSCLC cells. a Expression of miR-663a in 5 NSCLC cell lines (H1299, H3255, H460, H1395, H157) and normal bronchial cell line HBE135. miR-663a mimic significantly upregulated miR-663a level in H460 cells and its inhibitor downregulated miR-663a expression in H1299 cells. b Colony number of H460 cells transfected with miR-663a mimic was lower than cells transfected with control (control 596 ± 28 vs mimic 401 ± 18, p < 0.001). Colony number of H1299 transfected with miR-663a inhibitor was higher than cells transfected with control (control 171 ± 16 vs inhibitor 295 ± 18, p < 0.001). c miR-663a mimic increased G1 phase percentage and decreased S phase percentage in H460 cells. miR-663a inhibitor decreased G1 phase percentage and increased S phase percentage in H1299 cells. d Matrigel invasion assay showed that invading number of H460 transfected with miR-663a mimic was lower than cells transfected with control (control 86 ± 6 vs mimic 50 ± 3, p < 0.001). Invading number of H1299 cells transfected with miR-663a inhibitor was higher than cells transfected with control (control 84 ± 5 vs inhibitor 125 ± 9, p < 0.001). *p < 0.05, Error bars indicate standard deviation