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Fig. 5 | BMC Cancer

Fig. 5

From: Enhancement of the CXCL12/CXCR4 axis due to acquisition of gemcitabine resistance in pancreatic cancer: effect of CXCR4 antagonists

Fig. 5

Alteration of invasiveness of PaCa cells by CXCL12 stimulation and by co-culture with FB. The invasiveness of GEM-R and GEM-S MIA PaCa-2 cells was assessed by a double-chamber method using a Matrigel invasion assay system. PaCa cells were seeded into Matrigel pre-coated Transwell chambers. These cells were allowed to migrate for 22 h. The cells that invaded through the membrane to the bottom of the upper chamber were fixed, stained, photographed and counted. The number of invading cells was counted in 5 random microscopic fields (200×). a The invasion assay was performed in basal medium containing recombinant CXCL12 (100 ng/mL) (c) and co-cultured with FB. b The effect of CXCR4 antagonists (AMD11070 and KRH3955) on the invasiveness of GEM-R PaCa cells treated with GEM activated by CXCL12 (d) and co-culturing with FB was examined. Values are expressed as means ± SDs. Multiple comparisons were performed by using one-way ANOVA followed by Bonferroni test. **, P < 0.01; *, P < 0.05

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