Fig. 3

Alteration of CXCR4 mRNA and protein expression in MIA PaCa-2 cells by GEM. PaCa cells were treated with different concentrations of GEM (0 - 20 μM) for 24 h. a The CXCR4 mRNA levels in GEM-S and b in GEM-R were measured using RT-PCR (normalized to GAPDH expression). Values are expressed as means ± SD. Multiple comparisons were performed by using one-way ANOVA followed by Dunnett’s test. **, P < 0.01; *, P < 0.05 versus control (0 μM). c-f The expression of CXCR4 protein in GEM-S and g-j GEM-R was detected by immunostaining. CXCR4 (green) and DAPI (blue). Original magnification, ×200