Fig. 1

Effects of lenalidomide on the MM cell lines with variant levels of CRBN. a Expression of CRBN in MM cell lines. 100 μg of total proteins from whole cell lysates were loaded in each lane and probed with our recently developed mouse-anti-CRBN monoclonal antibody 2F11G5 (M-anti-CRBN) and mouse-anti-β-actin antibody (M-anti-β-Actin). b Comparison of CRBN expression in MM cell lines. The intensity of the β-actin band was considered as 1.0 and the relative intensity of CRBN band was compared with its corresponding β-actin band. c. The effects of lenalidomide on the MM cell lines. MM cells were plated in a volume of 200 μl at 10,000 cells per well in 96-well plate containing either DMSO (DMSO-treated control viabilities were considered as 100 %) or 10 μM lenalidomide. Cell survival was followed by MTT assay