Fig. 6

Glycolytic dependence predicts metabolic sensitivity to AIF ablation. Glucose consumption assay (Panel a): equal densities of cells were plated in fresh media, and total glucose was measured using the QuantiChrom™ Glucose Assay Kit (BioAssay Systems) 72 h after seeding. Total numbers of cells in each well were used to determine glucose consumption per cell. Sensitivity to glycolytic disruption (Panel b): Cells were seeded in equal densities and allowed to attach overnight before treatment with 50 mM 2-deoxyglucose. After 48 h cells were collected, and viability was determined by propidium iodide staining and flow cytometry. Data are shown as average ± standard deviation