Fig. 4

Contribution of AIF to the growth and migration of pancreatic cancer cells in vitro. Growth assay (Panel a): cells were plated in equal densities in replicate, harvested, and quantified by Coulter™ counting after 72 h of growth. Data are shown as average ± standard deviation. Scratch assay (Panel b): high densities of cells were seeded in replicate wells and allowed to attach for 12–36 h. A single scratch was made through the middle of each well, and width was assessed at 0 h (all cell lines), 6 h (HPAC), 10 h (HPAF-II), 24 h (PANC-1), and 48 h (BxPC-3 and MIA PaCa-2). Representative images are shown (Panel b); all images were captured at 10× magnification