Fig. 1

Representative microscopic acquisitions showing tumour cell proliferation assessed by Ki67 staining. Pictures are from tumour slices obtained from four different patients and analysed after 48 h of culture. Tumour slices were maintained in control conditions (a, c) or exposed to cetuximab (b, d). Ki67 immunostaining is shown in panels a and b. Automatic identification of stained nuclei is shown in panels c and d. The nuclei that are recognized as negative for Ki67-labelling are shown in blue, while those identified as positively stained are shown in orange. The four examples shown are representative of tumour areas with different proportions of proliferating cells, as defined by the % of Ki67 labelling (X 40 in a, b; X4 in c, d)