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Fig. 6 | BMC Cancer

Fig. 6

From: Selective activation of TNFR1 and NF-κB inhibition by a novel biyouyanagin analogue promotes apoptosis in acute leukemia cells

Fig. 6

Effects of FADD silencing on the antiproliferative and apoptotic properties of KC-53 in leukemic cells. a HL-60 and CCRF/CEM cells were transiently transfected with siRNA control or siRNA FADD followed by (i) immunoblotting for the detection of FADD protein levels and (ii) qPCR for measuring FADD mRNA levels. b Transfected cells were treated with 0 or 5 μΜ KC-53 for 24 h and cell viability was determined with the MTT assay. c Transfected cells were treated with 5 μΜ KC-53 for 6 h and protein levels were determined by immunoblotting. The results in panels a (i) and c are representative of three repetitions. The results in a (ii) and b panels represent the mean ± SEM of three replicates and are representative of three separate experiments. (***p value <0.001)

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