Fig. 2

Knockdown of S100A9 contributes to reducing OS proliferation, migration and invasion in vitro. a. Western blot was conducted to determine S100A9 protein in the three human OS cell lines (group1-blank control, group2-transfected with empty vectors, group3-transfected with siRNA-S100A9 vectors; n = 5, ^*p < 0.05; ^**p < 0.01, versus empty vectors group). b. Real-time Quantitative PCR was conducted to determine the mRNA levels of S100A9 in the three OS cell lines (n = 9, ^***p < 0.001, versus empty vectors group). c. CCK-8 was used to assess the three OS cell lines in 1, 2, 3 and 4 days (n = 15,^**p < 0.01;^***p < 0.001, versus empty vectors group at the same time points). d. Cell cycle distribution was tested in the group1, 2 and 3 using flow cytometry; the histogram of cell cycle distribution was shown (n = 5, ^**P < 0.01,^***P < 0.001, versus empty vectors group at G0/G1 phase; ^##p < 0.01, ^###p < 0.001, versus empty vectors group at S phase). e. Cell migration assay were evaluated by transwell chambers in group1, 2 and 3 (n = 5, ^***p < 0.001, versus empty vectors group). f. Cell invasion assay were evaluated by transwell chambers in group 1, 2 and 3 (n = 5,^***p < 0.001,versus empty vectors group)