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Fig. 4 | BMC Cancer

Fig. 4

From: Nemo-like kinase as a negative regulator of nuclear receptor Nurr1 gene transcription in prostate cancer

Fig. 4

Knockdown of NLK in LNCap cells upregulates Nurr1 promoter activity through both NF-κB- and CREB-binding sites in the Nurr1 promoter region. LNCaP cells were transfected with a shRNA vector targeting NLK and the three Nurr1 promoter luciferase constructs (wild-type or mutant), as indicated. 12 h after transfecting with WT-Nurr1 promoter constructs, cells were treated with DMSO (control), BAY 11–7082 (NF-κB inhibitor), KG-501 (CREB inhibitor) or ICG-001 (CBP inhibitor). Luciferase activity was measured 48 h after transfection. Results are plotted as means ± SD for three independent experiments. *p < 0.05, assessed by Student’s t-test. The illustrated numbers were ratios of data in each group to that in blank control

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