Fig. 3

NLK is involved in the suppression of Nurr1 expression in PCa cell lines. PC-3 cells were transfected with NLK-expressing or control vectors, and LNCaP cells were transfected with a shRNA vector targeting NLK or a control nonspecific shRNA vector. a 48 h after transfection, Western blot analysis was used to detect protein expression levels of NLK and Nurr1. b 48 h after transfection, real-time PCR was performed to detect mRNA levels of Nurr1. c Analysis of Nurr1 promoter-luciferase activity was used to determine Nurr1 transcriptional activity. Results are plotted as means ± SD for three independent experiments. *p < 0.05 (compared with control groups), assessed by Student’s t-test