Fig. 1

Expression of NLK and Nurr1 in human prostate cancer. a (I-IX): Paraffin-embedded tissue sections were stained with antibodies for NLK and Nurr1 and then counterstained with hematoxylin (×400). I-III: Negative controls for benign prostate gland and PCa specimens; IV: High NLK expression in benign prostate gland specimen; V: Medium NLK expression in low-grade PCa specimen; VI: Low NLK expression in high-grade PCa specimen; VII: Low Nurr1 expression in benign prostate gland specimen; VIII: Medium Nurr1 expression in low-grade PCa specimen; IX: High Nurr1 expression in high-grade PCa specimen. b Western blotting was performed to study the protein levels of NLK and Nurr1 in eight representative paired samples of PCa tissues (T) and non-tumorous adjacent tissues (N). c Western blot analysis of endogenous NLK and Nurr1 in BPH-1, PC-3 and LNCaP cells. Results are plotted as mean ± SD for three independent experiments. *p < 0.05, assessed by Student’s t-test