Fig. 5

Knockdown of CCNA2 and eEF2K inhibits NO production and iNOS expression in LPS-activated RAW264.7 cells. a RAW264.7 cells were transfected with CCNA2 and eEF2K siRNA at a final concentration of 40 nM for 48 h; mRNA levels were determined by qPCR and normalized to β-actin. b After 60 h, protein expression was determined by western blot; GAPDH served as the loading control. c RAW264.7 cells were transfected with CCNA2 and eEF2K siRNA at a final concentration of 40 nM; after 60 h, the cells were stimulated with 1 μg/ml LPS for 6 h. NO in the supernatant was measured using a nitrate/nitrite assay kit, and the values were normalized to total protein concentration. d iNOS mRNA expression was detected by qPCR and normalized to β-actin. The data are presented as the mean ± SD (n = 3) of three independent experiments. **p < 0.01; *p < 0.05