Fig. 3
LPS-activated RAW264.7-miR-125b cells promote 4T1 cell proliferation in vitro and in vivo. a 4T1 cells were cocultured with either RAW macrophage cells. Briefly, for coculture without cell-cell contact, 1 × 105 LPS-activated RAW264.7-miR-125b or RAW264.7-pLL3.7 cells were seeded in Boyden Transwell inserts. Transwells containing macrophages were then inserted into a 24-well plate and seeded with 3 × 105 4T1 tumor cells in each well. The cell viability of 4T1 cells was measured with MTS assay at different time points. Each bar represents the mean ± SD (n = 3) of three independent experiments. **p < 0.01; *p < 0.05. b-d. LPS-activated RAW264.7-miR-125b cells promote tumor growth in vivo. 4T1 cells and LPS-activated RAW264.7-miR-125b cells or LPS-activated RAW264.7-pLL3.7 cells were mixed at a ratio of 4:1 and then s.c. co-injected into 4- to 6-week-old BALB/c female mice. After 5 days, tumor length and width were measured with a caliper every 3 days for 3 weeks. The tumor volume at different time points is shown in panels b and c. Individual tumor weights and the average tumor weight at the experimental endpoint are shown in panels d. **p < 0.01; *p < 0.05