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Fig. 2 | BMC Cancer

Fig. 2

From: Oral proteasome inhibitor with strong preclinical efficacy in myeloma models

Fig. 2

Accumulation of ubiquitinated proteins and activation of apoptosis in cells. Lysates were prepared from RPMI8226 cells 4 h (a) or 24 h (b, c, d) after a 1-h treatment with 0.1 % DMSO or 500 nM of proteasome inhibitors. Western blot analysis was done with anti-Ubiquitin, anti-p21, anti-β-Catenin, anti-Hsp70, anti-phospho Hsp27 (S82), anti-Hsp27, anti-Gadd34, anti-caspase-8, and anti-caspase-9 antibodies. α-Tubulin was used as a loading control. Cleaved caspase-8 and 9 bands on the western blot were quantitated and expressed as a fold-increase over DMSO control

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