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Fig. 4 | BMC Cancer

Fig. 4

From: RETRACTED ARTICLE: CDK4 and miR-15a comprise an abnormal automodulatory feedback loop stimulating the pathogenesis and inducing chemotherapy resistance in nasopharyngeal carcinoma

Fig. 4

c-Myc binds to the promoter of miR-15a in NPC cells. a. c-Myc cDNA was transfected to 5–8 F and HONE1 NPC cells and its protein expression was examined by western blot. b. Overexpressed c-Myc reduced the expression of miR-15a in NPC cells. c. Crosslinked chromatin preparation from mock and pcDNA3.1-C-Myc transfected 5-8F and HONE1 cells was immunoprecipitated with anti-C-Myc. The c-Myc binding sites on the immunoprecipitated DNA was determined by PCR. Amplification of input chromatin (input) before immunoprecipitation was served as positive controls for chromatin extraction and PCR amplification. Chromatin immunoprecipitation using normal human IgG served as a negative control. The result indicated that overexpressed c-Myc could bind more miR-15a promoter region than that in control group in NPC cells. d. Luciferase reporter assay indicated that c-Myc could directly bind miR-15a promoter in NPC. (*P < 0.05)

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