Fig. 5
From: Epigenetic silencing of miR-181c by DNA methylation in glioblastoma cell lines
CTCF depletion results in increased promoter methylation and decreased expression of miR-181c. a Western-blot showing the levels of CTCF protein in cells infected by a lentiviral system expressing a shRNAi against CTCF. b Comparative DNA methylation analysis of the miR-181c promoter region. The percentage of methylated CpGs is shown for knockdown cells (U87MG-kd) and control cells (U87MG-ev). Black circles correspond to methylated CpGs and white circles to unmethylated CpGs. c primiR-181c expression levels measured by RT-qPCR with SYBR Green. d miR-181c expression levels measured by Taqman Assay. **p-value < 0.01