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Fig. 2 | BMC Cancer

Fig. 2

From: Epigenetic silencing of miR-181c by DNA methylation in glioblastoma cell lines

Fig. 2

DNA methylation of the miR-181c promoter region. a Scheme of the distribution of CpGs upstream the transcription start site and over the gene body of miR-181c. DNA methylation profile of miR-181c promoter region in different cell types. The analyzed region is demarcated by the black line upstream the transcription start site which represents a CpG island. A range of 8 to 12 independent clones were sequenced for each cell type. Black circles represent methylated CpGs; white circles represent non-methylated CpGs. The black triangles point to the CpGs that overlap with a CTCF binding motif and have a high level of methylation in T98G. b primiR-181c expression level in T98G cells and the T98G treated with 5-aza-2′-deoxycytidine, measured by RT-qPCR with SYBR Green. c miR-181c expression level in T98G cells and T98G treated with 5-aza-2′-deoxycytidine, measured with Taqman assay. *p-value < 0.05; **p-value < 0.01

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