Fig. 7

Pan-caspase inhibitor Z-VAD-FMK (Z-VAD) reduces CA IX shedding and expression in hypoxic HeLa cells, and recombinant CA IX ECD induces the Nanog pathway. a ELISA of the CA IX ECD shed from the whole population of the DOX and Z-VAD-FMK-treated cells. b qPCR analysis of the CA IX transcript level in the viable fraction of cells. DOX-surviving cells show higher ECD shedding and higher transcription of CA IX, whereas Z-VAD inhibits the ECD release and reduces CA IX transcription. c Cell-based dual luciferase reporter assay of HeLa cells incubated in the presence of recombinant rCA IX ECD showed alterations of several signal transduction pathways leading to changes in transactivation activities of the transcription factors indicated in the graph. d qPCR analysis confirmed the induction of Nanog gene transcription in the rCA IX ECD-treated cells. Error bars represent the mean ± SD. **p < 0.01, *p < 0.05