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Fig. 3 | BMC Cancer

Fig. 3

From: Apoptosis-induced ectodomain shedding of hypoxia-regulated carbonic anhydrase IX from tumor cells: a double-edged response to chemotherapy

Fig. 3

CA IX-negative CGL3 cells are more susceptible to apoptosis. a Representative FACS analysis of the CA IX expression in the sorted CA IX-negative (CA IX-) versus CA IX-positive (CA IX+) CGL3 cell subpopulations, and relative CA IX expression in the sorted subpopulations from four independent experiments. b Representative FACS analysis of the cell viability in the sorted subpopulations using double staining with the fluorescein di-acetate and propidium iodide. c Proportion of the viable, apoptotic and necrotic cells in the sorted subpopulations under control conditions and after treatment with CHX, as determined by FACS of FDA-PI staining in four independent experiments. CHX-treated CA IX cells showed significantly higher viability than CA IX- cells. Error bars represent the mean ± SD. **p < 0.01

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