Fig. 2

Quantitative analysis of MMP2, MMP9, TIMP1, TIMP2 and TIMP3 gene and protein expression. a Real-time RT-qPCR analysis of MMP2, MMP9, TIMP1, TIMP2 and TIMP3 gene expression in osteosarcoma biopsies from good and poor responders (n = 9 each). Expression levels were normalized to the expression of RPL19 (ribosomal protein L19) in the corresponding sample. b Western blot analysis of MMP2, MMP9, TIMP1, TIMP2 and TIMP3 protein expression in osteosarcoma biopsies from good and poor responders (n = 9 each). Response to preoperative chemotherapy according to the six-grade scale of Salzer-Kuntschik is indicated. c Densitometric quantification of the western blots shown in (b). The mean values ± SD of the analyzed good and poor responder groups are shown