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Fig. 6 | BMC Cancer

Fig. 6

From: Honokiol inhibits sphere formation and xenograft growth of oral cancer side population cells accompanied with JAK/STAT signaling pathway suppression and apoptosis induction

Fig. 6

Honokiol suppresses IL-6-mediated migration of SAS cells. a Two preparations of SAS cells were transfected with STAT3 siRNA (siSTAT3-1, siSTAT3-2) and the control siRNA group was transfected with the mismatch siRNA oligonucleotide. After 72 h, the STAT3 expression was determined by Western blot. b Wound healing assay. The STAT3 siRNA transfected SAS cells (siSTAT3-2) were seeded into a 6-well plate. After growing to confluence, straight scratches were made across the monolayer by using a white tip along plate cover. Then, IL-6 (50 ng/ml) or honokiol (5 μM) was added into wells as indicated and recorded by photography 24 h later. Honokiol inhibited the migration of SAS cells as potent as STAT3 siRNA. Notably, honokiol also suppressed the migration enhanced by IL-6. In this assay, honokiol (5 μM) and siSTAT3 did not affect the cell viability of these cells (Additional file 1: Figure S1)

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BMC Cancer

ISSN: 1471-2407

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