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Fig. 2 | BMC Cancer

Fig. 2

From: Honokiol inhibits sphere formation and xenograft growth of oral cancer side population cells accompanied with JAK/STAT signaling pathway suppression and apoptosis induction

Fig. 2

SP-derived spheres from SAS and OECM-1 cell lines possess the stemness properties. a After cultured in an anchorage-independent manner for 7 days, the spheroidal morphology (phase-contrast images) of SAS (left) and OECM-1 (right) sphere cells were distinct from those of parental cells. b Marked higher expression of stemness markers in SAS and OECM-1 sphere (“S”) cells compared to parental (“P”) cells. The expression of various stemness markers was analyzed by RT-PCR and GAPDH was used as a loading control. The intensities of the PCR bands were quantified by densitometry. The densitometric values indicated at the top of the bands are expressed relative to the value of parental cells after being normalized to actin (#: The intensity of ABCG2 band of parental SAS cells was undetectable in this PCR condition). Both the SAS and OECM-1 sphere cells had higher capacities in sphere (c) and colony (d) formation than parental cells. Data are shown as mean ± SD from experiments performed in triplicates. *, p < 0.05; **, p < 0.01

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BMC Cancer

ISSN: 1471-2407

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