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Fig. 2 | BMC Cancer

Fig. 2

From: TNF-alpha promotes lymphangiogenesis and lymphatic metastasis of gallbladder cancer through the ERK1/2/AP-1/VEGF-D pathway

Fig. 2

VEGF-D mRNA transcription and protein expression in three GBC cell lines after treatment with TNF-α. GBC cells (NOZ, GBC-SD, and SGC-996) were treated with varying concentrations of TNF-α (10, 20, 50 and 100 ng⁄ mL) for 12 or 24 h. The VEGF-D mRNA and protein levels were measured by real-time PCR (a, b) and ELISA (c), respectively, and increased in a dose- and time-dependent manner in NOZ and GBC-SD cell lines but not SGC-996 cells. (*P < 0.05; **P < 0.01; ***P < 0.001)

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