Fig. 5
MiR-29c inhibits the growth of nasopharyngeal carcinoma cells HNE1 and CNE2 by MTT assay. Cells were transformed with pre-miR-29c and cultured for 24 h. 5 × 104 cells were seeded into 96-well dish triplicately. 20ul MTT solution was added to each well and then 200ul DMSO was added to the well with cells. Read optical density at 490 nm and subtract background at 570 nm. The readout was recorded at 4 time points (24, 48, 72, 96,120 h)