Fig. 4

The expression of BDNF mRNA variants respectively containing exons IV, VI and IX in HBE, SK, LK2, A549 and LTE cells by RT-PCR. BDNF mRNA levels remained invariable in variants containing exons IV and VI. SK and LK2 cells showed much higher level of BDNF mRNA variant containing exon IX, compared with HBE, A549 and LTE cells. β-actin was used as a loading control to assure equal amounts of protein in all lanes. The experiments for each cell line were repeated at least three times