Table 1 Sequences of polymerase chain reaction primers and probes for HPV-16 specific DNA and transcript
A. End-point PCR primers for the detection and amplification of HPV-16 specific DNA HPV-16 E6/E7 forward primer: 5’ -CCCAGCTGTAATCATGCATGGAGA-3’ reverse primer: 5’ -GTGTGCCCATTAACAGGTCTTCCA-3’ |
β-globin forward primer: 5’ -CAACTTCCACGGTTCACC-3’ reverse primer: 5’ -GAAGAGCCAAGGACAGGTAC-3’ |
B. Quantitative PCR primers for the detection and amplification of HPV-16 specific DNA HPV-16 E7 forward primer: 5’ -GATGAAATAGATGGTCCAGC-3’ reverse primer: 5’ -GCTTTGTACGCACAACCGAAGC-3’ |
C. End-point RT-PCR primers for the detection and amplification of HPV-16 specific transcript HPV-16 E6 forward primer: 5’ -CAGGAGCGACCCAGAAAGTT-3’ reverse primer: 5’ -GCAGTAACTGTTGCTTGCAGT-3’ GAPDH forward primer: 5’ -TTGCCCTCAACGACCACTTT-3’ reverse primer: 5’ -TTGCCCTCAACGACCACTTT-3’ |
D. Taqman probes for the detection and amplification of HPV-16 specific transcript HPV-16 E6/E7 forward primer: 5’ -(MGB)-CCAGCTGTAATCATGCATGGA-3’ reverse primer: 5’ -(MGB)-CAGTTGTCTCTGGTTGCAAATCTAA-3’ |