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Table 1 Sequences of polymerase chain reaction primers and probes for HPV-16 specific DNA and transcript

From: A pilot study to compare the detection of HPV-16 biomarkers in salivary oral rinses with tumour p16INK4a expression in head and neck squamous cell carcinoma patients

A. End-point PCR primers for the detection and amplification of HPV-16 specific DNA
HPV-16 E6/E7
forward primer: 5’ -CCCAGCTGTAATCATGCATGGAGA-3’
reverse primer: 5’ -GTGTGCCCATTAACAGGTCTTCCA-3’
β-globin
forward primer: 5’ -CAACTTCCACGGTTCACC-3’
reverse primer: 5’ -GAAGAGCCAAGGACAGGTAC-3’
B. Quantitative PCR primers for the detection and amplification of HPV-16 specific DNA
HPV-16 E7
forward primer: 5’ -GATGAAATAGATGGTCCAGC-3’
reverse primer: 5’ -GCTTTGTACGCACAACCGAAGC-3’
C. End-point RT-PCR primers for the detection and amplification of HPV-16 specific transcript
HPV-16 E6
forward primer: 5’ -CAGGAGCGACCCAGAAAGTT-3’
reverse primer: 5’ -GCAGTAACTGTTGCTTGCAGT-3’
GAPDH
forward primer: 5’ -TTGCCCTCAACGACCACTTT-3’
reverse primer: 5’ -TTGCCCTCAACGACCACTTT-3’
D. Taqman probes for the detection and amplification of HPV-16 specific transcript
HPV-16 E6/E7
forward primer: 5’ -(MGB)-CCAGCTGTAATCATGCATGGA-3’
reverse primer: 5’ -(MGB)-CAGTTGTCTCTGGTTGCAAATCTAA-3’