Skip to main content

Table 2 Characteristics of antibodies used

From: Clinical significance of AGE-RAGE axis in colorectal cancer: associations with glyoxalase-I, adiponectin receptor expression and prognosis

Antibody Supplier/Company Methods of antigen retrieval AB dilution Conditions of incubation
Anti-AGE TransGenic Microwave 20 min 1:500 Overnight
(monoclonal mouse)   Citrate buffer (pH6)   4 °C
Anti-RAGE R&D Microwave 20 min 1:500 Overnight
(polyclonal goat)   Citrate buffer (pH6)   4 °C
Anti-Glyoxalase-I Santa Cruz Microwave 20 min 1:300 Overnight
(monoclonal mouse) Biotechnology Citrate buffer (pH6)   4 °C
Anti-Adiponectin Receptor Type 1 Phoenix Microwave 20 min 1:500 60 min
(polyclonal rabbit) Pharmaceuticals Citrate buffer (pH6)   4 °C
Anti-Adiponectin Receptor Type 2 Phoenix Microwave 20 min 1:500 Overnight
(polyclonal rabbit) Pharmaceuticals Citrate buffer (pH6)   4 °C