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Fig. 2 | BMC Cancer

Fig. 2

From: Cathepsin K induces platelet dysfunction and affects cell signaling in breast cancer - molecularly distinct behavior of cathepsin K in breast cancer

Fig. 2

The influence of cat K (20 nM), papain (1.6 µM), and cat L (0.2 µM) on the effect of α-thrombin. (a) The results show platelet aggregation induced by cat K (20 nM) followed by α-thrombin stimulation (1.0 UNIH/500 µL) after 2 min. α-Thrombin stimulation was slightly prolonged (black line). Cat K does not interfer with α-thrombin activity on platelets aggregation (blue line). Data are expressed as mean ± SEM from 6 independent experiments (*p<0.05). (b) Papain (1.6 µM) followed by α-thrombin stimulation (1.0 UNIH/500 µL) (blue line). The inverse stimulation (α-thrombin followed by papain (1.6 µM) and cat L (0.2 µM)), (black and orange lines). The bar graph shows percentage of aggregation expressed as mean ± SEM from 3 independent experiments (**p<0.01, ***p<0.001). (c) Representative traces showing typical aggregation induced by cat K (20 nM), papain (1.6 µM), and α- thrombin (1.0 UNIH/500 µL). The arrows indicate when agonists were added. (d) Stimulation by papain (1.6 µM) followed by cat K (20 nM), red line. Inverse cat K stimulation (20 nM) followed by papain (1.6 µM), black line. (e) The bar graph shows percentages of aggregation. Data are expressed as mean ± SEM from 3 independent experiments (*p<0.05)

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