Fig. 4

The topographical distributions of private mutations on microscope slides can also distinguish public from private mutations. a Public mutations are detected in most microdissected regions (yellow circles) but a private mutation found on both tumor sides is present in only some small regions (blue circles) in Cancer N. b Mutation topographic distributions on microscope slides can distinguish public from private mutations using a 60 % detection threshold (dotted line). Public mutations found in only some small areas (red circles) may be secondary to subsequent LOH. The high proportions of microdissected areas positive for the private mutations found on both sides in Cancers N and M (blue circles) may reflect that even immediately adjacent glands may have different private mutations in these well-mixed cancers