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Fig. 4 | BMC Cancer

Fig. 4

From: Cyclopamine tartrate, an inhibitor of Hedgehog signaling, strongly interferes with mitochondrial function and suppresses aerobic respiration in lung cancer cells

Fig. 4

CycT treatment increases ROS production in NSCLC H1299 (a), A549 (b), and H460 (c) cells. d SANT1 treatment also increases ROS production in NSCLC H1299 cells. NSCLC cells were treated with CycT or SANT1 for the indicated time periods. Then cells were incubated with 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) for 30 min. Fluorescence intensity was measured and normalized according to cell density. For statistical analysis, the levels in CycT-treated cells were compared to the levels in untreated cells, by using Welch 2-sample t-test. *, p value < 0.05; **, p value < 0.005

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