Fig. 4

CycT treatment increases ROS production in NSCLC H1299 (a), A549 (b), and H460 (c) cells. d SANT1 treatment also increases ROS production in NSCLC H1299 cells. NSCLC cells were treated with CycT or SANT1 for the indicated time periods. Then cells were incubated with 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) for 30 min. Fluorescence intensity was measured and normalized according to cell density. For statistical analysis, the levels in CycT-treated cells were compared to the levels in untreated cells, by using Welch 2-sample t-test. *, p value < 0.05; **, p value < 0.005