Fig. 4
Northern blot analysis of RNA isolated from A2780 cells treated with docetaxel. A2780 cells were treated with 0.2 μM docetaxel for 48 h and total RNA was isolated. RNA was resolved by agarose gel electrophoresis and transferred to PVDF membranes for hybridization with the 32P-end-labeled oligonucleotide probe, 28S-5. a The panel on the left shows the agarose gel and the panel on the right shows the Northern blot of the gel. RNA bands are indicated by arrows with the size of the band in nucleotides alongside. b A schematic diagram of the 28S rRNA sequence showing conserved and variable regions, based on the structure of the 28S rRNA as defined by Gorski et al. (1987) [53] and Wakeman et al. [28]. Location of the probes in the 28S rRNA sequence is shown above the diagram using arrows and the location of the cleavage sites and resulting bands are shown below the diagram