Fig. 5

Gemcitabine treatment of ampullary cancer cell lines. Cell viability was quantified by MTT assay. a baseline cell growth relative to the fastest growing (MDA-AMP7) cells (b) cell growth in CAF-CM relative to controls (c) cell growth under low-dose Gemcitabine (40 nM) treatment relative to PANC-1 (d) cell viability under varying concentration of Gemcitabine (100 nM, 1 μM or 10 μM) relative to untreated controls. Bar charts depict mean and error bars standard deviation from n = 6 measurements, * p < 0.05 in two-sided t-test compared to untreated control. Abbreviations: MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, CAF-CM cancer associated fibroblast conditioned medium