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Fig. 6 | BMC Cancer

Fig. 6

From: The anti-oxidative transcription factor Nuclear factor E2 related factor-2 (Nrf2) counteracts TGF-β1 mediated growth inhibition of pancreatic ductal epithelial cells -Nrf2 as determinant of pro-tumorigenic functions of TGF-β1

Fig. 6

Nrf2 modulates MAPK and Smad signaling in benign, premalignant and malignant pancreatic ductal epithelial cells. a H6c7-pBp and H6c7-kras cells were transfected with a control vector (pcDNA3.1) or Nrf2-HA or (b) H6c7-pBp, H6c7-kras and Colo357 cells were transfected either with control siRNA or Nrf2 siRNA. Then, cells were either left untreated or were treated with 10 ng/ml TGF-β1 for 48 h. Representative western blots of 3-4 independent experiments showing expression of phosphorylated and total p38 (p-/t-p38), phosphorylated Smad3 (p-Smad3, marked by arrow) and total-Smad2/3 (t-Smad3 marked by arrow), phosphorylated and total Erk (p-/t-Erk) and p21. Hsp90 was detected as loading control. Numbers above each band indicate average band intensities determined by densitometry. Values of the proteins of interest were divided by the values of the corresponding loading control (Hsp90)

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