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Fig. 3 | BMC Cancer

Fig. 3

From: The anti-oxidative transcription factor Nuclear factor E2 related factor-2 (Nrf2) counteracts TGF-β1 mediated growth inhibition of pancreatic ductal epithelial cells -Nrf2 as determinant of pro-tumorigenic functions of TGF-β1

Fig. 3

Nrf2 antagonizes the growth inhibiting effect of TGF-β1 on pancreatic ductal epithelial cells. a H6c7-pBp and H6c7-kras cells that were transfected with a control vector (pcDNA3.1) or Nrf2-HA and left untreated or were treated with 10 ng/ml TGF-β1 for 48 h. Determination of vital cell numbers by trypan blue exclusion and cell counting. Verification of Nrf2 overexpression was performed by western blotting using an HA-specific antibody and Hsp90 as loading control. b H6c7-pBp, H6c7-kras and Colo357 cells were transfected either with control siRNA or Nrf2 siRNA and left untreated or were treated with 10 ng/ml TGF-β1 for 48 h. Determination of vital cell numbers by trypan blue exclusion and cell counting. Verification of Nrf2 knockdown was performed by western blotting using a t-Nrf2 antibody and Hsp90 as loading control. Data of vital cell numbers are presented as Box-Plots of 4-6 independent experiments. * = p < 0.05

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