Fig. 6
From: Targeting Rad50 sensitizes human nasopharyngeal carcinoma cells to radiotherapy
Ad-RAD50 abrogated the DSBs-induced G2/M arrest and enhances IR-induced DNA damage. a Ad-RAD50 infection combining with IR decreased the p-cdc25c and p-cdk1-Y15 expression compared with IR alone or Ad-EGFP infection combining with IR. b DNA DSBs in CNE1 and 5–8 F cells detected by neutral comet assay at 24 h after IR, respectively. Original magnification: ×40. Damaged and fragmented DNA migrates toward the anode, producing a comet tail. The mean olive tail movements (MTMs) measured by using CASP software. MTM values ± SEM are shown. Ad-RAD50 combined with 4Gy IR significantly increased DSBs compared to all the other groups (* P < 0.05, ** P < 0.01)