Fig. 3

The binding interaction between Rad50 and Mre11/mutant Rad50. a and b are the confocal assays of Ad-RAD50 infected CNE1 cells, c and d the confocal assays of Ad-RAD50-treated CNE1 cells after IR. In (a) and (c), the overlap of the signals corresponds to wild-type Rad50 (blue) and Mre11 (red). In (b) and (d), the overlap of the signals corresponds to wild-type Rad50 (blue) and mutant Rad50 (red), which suggests a binding interaction between these proteins. e and f The co-IP of wild-type Mre11 and Rad50 in CNE1 cells. The Ad-RAD50-treated cells demonstrated significant inhibition of the interaction with wild-type Mre11 with Rad50 and Nbs1. Cells with Ad-EGFP treatments were used as controls