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Fig. 2 | BMC Cancer

Fig. 2

From: Transketolase-like 1 ectopic expression is associated with DNA hypomethylation and induces the Warburg effect in melanoma cells

Fig. 2

TKTL1 is expressed in metastatic melanoma cell lines and is regulated by promoter hypomethylation. a Using qRT-PCR the expression of TKTL1 in two melanoma cell lines, LM-MEL-59 and LM-MEL-44 was determined. b TKTL1 expression in LM-MEL-59 and LM-MEL-44 was determined by IHC. Original magnification, 20×. c Bisulfite conversion and MS-qPCR was performed to assay the promoter methylation status of TKTL1 in melanocytes and metastatic melanoma cells. Relative levels of methylated and unmethylated products (M/UM) was quantified. d QRT-PCR was employed to detect changes in expression of TKTL1 by 5-azacytidine treatment in LM-MEL-44 cell line. Values are ± SD of three independent experiments in triplicate (*, p < 0.05)

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