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Fig. 3 | BMC Cancer

Fig. 3

From: METCAM/MUC18 is a novel tumor and metastasis suppressor for the human ovarian cancer SKOV3 cells

Fig. 3

Effects of huMETCAM/MUC18 expression on the in vivo tumorigenesis of SK-OV-3 clones/cells at the SC injection sites. a Tumorigenicity of the METCAM clone 2D and the Control (Vector) clone 3D of SK-OV-3 was determined by subcutaneous injection of 5 × 106 cells of cells from each clone at the dorsal and ventral sides in female athymic nude mice. Tumor proliferation by the two clones is shown by plotting mean tumor volumes/weights versus time after injection. P values were determined by analyzing all the data with the student’s t test by using 1-tailed distribution-type 1 method. P values between tumor volumes through the time course of the METCAM clone 2D and that of the control (vector) clone 3D were 0.0142 at the dorsal site and 0.025 for the ventral site of injection, respectively. P value between the dorsal and the ventral sites of the METCAM clone 2D was 0.024 (**) and that between the two sites of the control (vector) clone 3D was 0.016 (*). b The panels a and b show the mice bearing tumors from the METCAM clone 2D and the control (vector) clone 3D, respectively, at the dorsal sites (DSC). The panels c and d show the mice bearing tumors from the METCAM clone 2D and the control (vector) clone 3D, respectively, at the ventral sites (VSC). c The mean final tumor weights of the two clones injected at both dorsal and ventral sites in athymic nude mice were compared at the endpoint. Both the mean final tumor weights from five mice of the control (vector) clone 3D were statistically significantly heavier than the mean tumor weight from those of the METCAM clone 2D, since the P values, which were analyzed by the Student’s t test (one-tailed distribution-type 1 method) between the tumors from the METCAM clone 2D and the control (vector) clone 3D at the dorsal and ventral sites were 0.0008 and 0.0022, respectively. The P values of the final tumor weights analyzed by the Student’s t test (one-tailed distribution-type 1 method) between the dorsal and ventral sites were 0.047 for the METCAM clone 2D and 0.05 for the control (vector) clone 3D, respectively

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