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Fig. 9 | BMC Cancer

Fig. 9

From: Chronic ethanol feeding promotes azoxymethane and dextran sulfate sodium-induced colonic tumorigenesis potentially by enhancing mucosal inflammation

Fig. 9

Chronic ethanol feeding promotes AOM/DSS-induced elevation of Ki67 in mouse colon. AOM/DSS-induced colonic tumorigenesis was induced with (EF) or without (PF) 4 % ethanol feeding as described in Methods section. At the pre-cancerous stage (Day 44; 2 week after second course of DSS colitis), colonic sections were stained for Ki67 (red) and F-actin (green) (a). Red fluorescence density in Ki67-positive cells was measured by densitometry (b). Fluorescence values are mean ± SEM (n = 5; each n is average of 8–10 cells in each section). Asterisks indicate the values that are significantly (p < 0.05) different from corresponding PF values. Hash tag symbols indicate the values that are significantly (p < 0.05) different from corresponding “Control” values. Colonic sections were also co-stained for F-actin (green) and activated caspase-3 (red) (c)

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