Fig. 8
Chronic ethanol feeding modulates gene expression of tight junction proteins in the colon of AOM/DSS treated mice. AOM/DSS-induced colonic tumorigenesis was induced with (EF) or without (PF) 4 % ethanol feeding as described in Methods section. a-c At the pre-cancerous stage (Day 44; 2 week after second course of DSS colitis), qRT-PCR analysis performed in RNA from distal colonic mucosa for occludin (a), ZO-1 (b) and claudin-3 (c). Values are mean ± SEM (n = 4). Asterisks indicate the values that are significantly (p < 0.05) different from corresponding PF values. Hash tag symbols indicate the values that are significantly (p < 0.05) different from corresponding “Control” values. d Cryosections of colon were stained for occludin (green) and ZO-1 (red)