Fig. 6

Chronic ethanol feeding promotes cytokine and chemokine gene expression in AOM/DSS-induced colonic tumorigenesis. AOM/DSS-induced colonic tumorigenesis was induced with (EF) or without (PF) 4 % ethanol feeding as described in Methods section. At the pre-cancerous stage (Day 44; 2 week after second course od DSS colitis), qRT-PCR analysis performed in RNA from distal colonic mucosa for IL-6 (a), IL-1α (b), TNFα (c), CCL5/RANTES (d), CXCL9/MIG (e) and CXCL10/IP-10 (f). Values are mean ± SE (n = 7). Asterisks indicate the values that are significantly (p < 0.05) different from corresponding PF values. Hash tag symbol indicates the value that is significantly (p < 0.05) different from corresponding “Control” value