Fig. 1

Ethanol feeding increases AOM/DSS-induced tumorigenesis in mouse colon. a The scheme shows one time administration of azoxymethane (AOM) followed by three course of DSS (dextran sodium sulfate)-induced colitis and separated by 15-day recovery periods. Colon was examined 30 days after the third course of DSS colitis. During all recovery periods, animals were fed liquid diet with or without 4 % ethanol (EtOH) or isocaloric maltodextrin. b Body weights were recorded twice a week. Values represent the mean of 7–9 samples per group. c The number of tumors per colon was counted and the tumor size evaluated by measuring the diameter in AOM/DSS-treated mice with (EF) or without (PF) EtOH feeding. Values for total tumors per colon and tumors of 3 mm or greater diameter are mean ± SEM (n = 6 for PF and 7 for EF). Asterisks indicate the values that are significantly different (p < 0.05) from corresponding values for PF group