Fig. 6

Rac1 signaling is critical for migration of cells expressing gBΔR. a HeLa cells stably expressing gBΔR in 24 well plates when 80-90 % confluent were scratched with a 1000 μl pipette tip. After the scratch was performed, the cells were incubated with medium or medium supplemented with 50 μM of NSC23766 or vehicle (PBS). Wound closure was monitored at 16 h post scratch and imaged using a confocal microscope (Magnification, x 20 objective). A representative image of cell migration is provided. b The open area (scratch) was quantified with TSratch software and the data represented as a histogram. c Data from Transwell migration assays showing alterations on the migration of HeLa cells expressing gBΔR through a permeable membrane in response to incubating them with Rac1 inhibitor and the vehicle for the inhibitor (PBS) is depicted. Columns with asterisk mark (panels b and c) denotes the value to be statistically significant (p < 0.05) by LSD