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Fig. 4 | BMC Cancer

Fig. 4

From: KSHV gB associated RGD interactions promote attachment of cells by inhibiting the potential migratory signals induced by the disintegrin-like domain

Fig. 4

HeLa cells expressing gBΔR induce exaggerated changes at the edges of the cell membranes. a HeLa cells stably expressing gB, gBΔD, and gBΔR were fixed, permeabilized, and stained for polymerized actin by rhodamine-labeled phalloidin for 20 min at room temperature, washed, mounted using a mountant with DAPI prior to examining under a Nikon microscope using appropriate filters. b In a separate experiment, the cells expressing gBΔR were either treated with medium supplemented with 50 μg/ml of pre-immune IgGs or anti-DLD IgGs; or treated with 50 μM NSC23766 (Rac1 inhibitor) or 1 μM cyto-D for 2 h, prior to staining as above and viewing under the fluorescent microscope. A representative figure of the cells under different conditions is provided at a magnification of x 100. Closed yellow arrow heads, and arrows denote filopodial and lamelipodia, respectively. c Filopodial quantification: 300-500 μm long filopodial structures were counted on ten randomly selected cells for each treatment and the numbers were averaged. To avoid bias, the counting of filopodial projections for each treatment was performed by two different individuals and averaged. Each point on the plot represents the average from three different experiments. Asterisks on the data point denote the value to be statistically significant (p˂0.05) by LSD

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