Fig. 6

MG glycates M2 insert of PKM2 enzymes. Purified protein complex from EAC was treated with 1 mM MG for 5 days at room temperature. MG treated or untreated samples were run on 7.5–15 % SDS-PAGE gel followed by mass spectroscopy analysis for PKM2. Area nearby glycated peptide’s peak is magnified. Red arrow shows glycation at Arg 399 residue in M2 insert of PKM2 whereas black arrow for nonglycation of the peptide of 17 aa