Fig. 5

MG inhibits GAPDH activity. Enzymatic activity of GPI and GAPDH in the extracts of 3MC induced tumor tissues (a) and EAC cells (b). Activity of both enzymes was measured in the absence and presence of 0.5 and 1 mM MG at 25 °C for 10 mins. Percent inhibition was calculated considering the activity of enzyme in absence of MG as 100 % (vehicle). Data presented as means ± SD from three independent experiments. **p < 0.01 vehicle vs 0.5 mM or 1 mM MG