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Fig. 3 | BMC Cancer

Fig. 3

From: Molecular association of glucose-6-phosphate isomerase and pyruvate kinase M2 with glyceraldehyde-3-phosphate dehydrogenase in cancer cells

Fig. 3

Both PKM2 and GPI interact with GAPDH. a Immunoprecipitate of GAPDH antibody was subjected to immunoblot with PKM2 (panel 1), GPI (panel 2), GAPDH (panel 3) or mouse IgG heavy chain (panel 4) specific antibody. b Immunoprecipitate of PKM2 was subjected to immunoblot with PKM2, GPI, GAPDH or rabbit IgG heavy chain specific antibody. c Immunoprecipitate of GPI was subjected to immunoblot with PKM2, GPI, GAPDH or mouse IgG heavy chain specific antibody. Note that PKM2 was not immunoprecipitated with GPI antibody and vice versa. Mouse IgG was used as negative control for immunoprecipitation of GAPDH and GPI antibodies, and rabbit IgG for PKM2 (lanes 3 and 4). Antibodies against heavy chain of mouse IgG and rabbit IgG were used as loading controls for immunoblots (panel 4)

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BMC Cancer

ISSN: 1471-2407

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