Fig. 3
In vivo growth characteristics of MB3W1. a. 5 × 104 luciferase-transduced MB3W1 cells were xenografted either infra- (open circles) or supratentorially (closed circles), and tumor growth was monitored by BLI. b. Survival curves after infra- (pink, n = 6) versus supratentorial inoculation (blue, n = 5; one animal lost during surgery (censored)); grey: untreated controls, surgery only (n = 2). c. Survival curves of mice inoculated with untransduced tumor cells: comparison of MB3W1 cells previously grown in different culture conditions: supratentorial inoculation of cells previously grown as spheres in serum-free medium (pink curves, n = 5) and compared to inoculation of cells previously grown in serum-containing medium – cells were only derived from the non-proliferative adherent fraction - (blue curves, n = 5). Circles: 5 × 105 cells, squares: 5 × 104 cells, triangles: 5 × 103 cells. d. Growth characteristics of CD133+ (closed triangles, n = 5) and CD133− (open triangles, n = 5) MB3W1 cells after supratentorial inoculation of 5000 tumor cells